Production, partial characterization and antioxidant properties of exopolysaccharide α-d-glucan produced by KC117496 isolated from an idli batter.

Journal: Journal of food science and technology
Published Date: Nov 8, 2018

Abstract

Exopolysaccharide (EPS) produced from KC117496 isolated from the naturally fermented batter has been reported earlier. Here, study aimed to optimize the carbon source to enhance the yield of EPS production, partial characterization and antioxidant activity of α-d-glucan EPS. Among different disaccharides (sucrose, maltose, lactose) and monosaccharides (glucose, galactose and fructose), combination of sucrose and glucose at 2% showed highest EPS production of 4.55 g/L in MRS medium. The molecular weight was identified as ~ 4.428 × 10 kDa with MALDI-TOF mass spectroscopy. 1D and 2D NMR results exhibited the presence of α-1-6 and α-1-3 linked glucose revealed EPS as a glucan. Antioxidant properties of glucan (500 µg/mL) revealed the significant oxidation alleviation potential such as DPPH (74%) and Hydroxyl radical activity (97.8%), whereas metal chelating activity (70%) was lower as compare to control standard. These characteristics of glucan EPS reveals its potential application in food and pharmaceutical industry.

Authors

  • Chinnashanmugam Saravanan
    Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.
  • Digambar Kavitake
    Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.
  • Sujatha Kandasamy
    Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.
  • Palanisamy Bruntha Devi
    Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.
  • Prathapkumar Halady Shetty
    Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.

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