Aflatoxin B Degradation and Detoxification by CG1061 Isolated From Chicken Cecum.

Journal: Frontiers in pharmacology
Published Date: Jan 17, 2019

Abstract

Aflatoxin B (AFB) is one of the most hazardous mycotoxins contamination in food and feed products, which leads to hepatocellular carcinoma in humans and animals. In the present study, we isolated and characterized an AFB degrading bacteria CG1061 from chicken cecum, exhibited an 93.7% AFB degradation rate by HPLC. 16S rRNA gene sequence analysis and a multiplex PCR experiment demonstrated that CG1061 was a non-pathogenic . The culture supernatant of CG1061 showed an 61.8% degradation rate, whereas the degradation rates produced by the intracellular extracts was only 17.6%, indicating that the active component was constitutively secreted into the extracellular space. The degradation rate decreased from 61.8 to 37.5% when the culture supernatant was treated with 1 mg/mL proteinase K, and remained 51.3% when that treated with 100°C for 20 min. We postulated that AFB degradation was mediated by heat-resistant proteins. The content of AFB decreased rapidly when it was incubated with the culture supernatant during the first 24 h. The optimal incubation pH and temperature were pH 8.5 and 55°C respectively. According to the UPLC Q-TOF MS analysis, AFB was bio-transformed to the product CHO and other metabolites. Based on the results of experiments on chicken hepatocellular carcinoma (LMH) cells and experiments on mice, we confirmed that CG1061-degraded AFB are less toxic than the standard AFB. CG1061 isolated from healthy chicken cerum is more likely to colonize the animal gut, which might be an excellent candidate for the detoxification of AFB in food and feed industry.

Authors

  • Lingling Wang
    Department of Neurology, Shanghai Pudong New Area People's Hospital, Shanghai, China.
  • Jun Wu
    Department of Emergency, Zhuhai Integrated Traditional Chinese and Western Medicine Hospital, Zhuhai, 519020, Guangdong Province, China. quanshabai43@163.com.
  • Zhiwen Liu
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Yutao Shi
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Jinqiu Liu
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Xiaofan Xu
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Shuxian Hao
    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
  • Peiqiang Mu
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Fengru Deng
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Yiqun Deng
    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.

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