Reducing Quorum Sensing-Mediated Virulence Factor Expression and Biofilm Formation in by Using the Potential Quorum Sensing Inhibitor L-Carvone.

Journal: Frontiers in microbiology
Published Date: Jan 9, 2019

Abstract

Quorum sensing (QS), one of the most remarkable microbiological discoveries, is considered a global gene regulatory mechanism for various traits in bacteria, including virulence and spoilage. , an opportunistic pathogen and a dominant psychrophile, uses the -type QS system to regulate the production of virulence factors and biofilms, which are harmful to the food industry. Based on the QS interference approach, this study aimed to reveal the efficacy of L-carvone at sublethal concentrations on QS-regulated virulence factors and biofilm formation in QS inhibitory activity was demonstrated by the reduction in swinging motility (61.49%), swarming motility (74.94%), biofilm formation (52.41%) and acyl-homoserine lactone (AHL) production (0.5 μL/mL). Additionally, analysis and RT-qPCR studies for AHL synthase HalI and QS transcriptional regulator HalR revealed a plausible molecular mechanism for QS inhibition by L-carvone. These findings suggest that L-carvone (a main component of spearmint essential oils) could be used as a novel quorum sensing inhibitor to control in the food industry.

Authors

  • Tingting Li
    Key Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, Dalian, China.
  • Yongchao Mei
    College of Food Science and Technology, Bohai University, Jinzhou, China.
  • Binbin He
    College of Food Science and Technology, Bohai University, Jinzhou, China.
  • Xiaojia Sun
    College of Food Science and Technology, Bohai University, Jinzhou, China.
  • Jianrong Li
    College of Food Science and Technology, Bohai University, Jinzhou, China.

Keywords

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