Quorum Sensing System of Rm01 Controls Lipase and Biofilm Formation.

Journal: Frontiers in microbiology
Published Date:

Abstract

Quorum sensing (QS) promotes extracellular enzyme (EE) activity via the exogenous signal -acylhomoserine lactone (AHL), which facilitates marine particle degradation, but the species that engage in this regulatory mechanism remain unclear. Here, we obtained AHL-producing and AHL-degrading strains from marine particles. The strain Rm01 of the group (RBG), which was capable of both AHL producing and degrading, was chosen to represent these strains. We demonstrated that Rm01 possessed a complex QS network comprising AHL-based QS and quorum quenching (QQ) systems and autoinducer-2 (AI-2) perception system. Rm01 was able to respond to multiple exogenous QS signals through the QS network. By applying self-generated AHLs and non-self-generated AHLs and AI-2 QS signal molecules, we modulated biofilm formation and lipase production in Rm01, which reflected the coordination of bacterial metabolism with that of other species via eavesdropping on exogenous QS signals. These results suggest that might be one of the participators that could regulate EE activities by responding to QS signals in marine particles.

Authors

  • Ying Su
    College of Marine Life Science, Ocean University of China, Qingdao, China.
  • Kaihao Tang
    College of Marine Life Science, Ocean University of China, Qingdao, China.
  • Jiwen Liu
    College of Marine Life Science, Ocean University of China, Qingdao, China.
  • Yan Wang
    College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
  • Yanfen Zheng
    College of Marine Life Science, Ocean University of China, Qingdao, China.
  • Xiao-Hua Zhang
    College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.

Keywords

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