Colorimetric Analysis of Carcinoembryonic Antigen Using Highly Catalytic Gold Nanoparticles-Decorated MoS Nanocomposites.

Journal: ACS applied bio materials
Published Date: Dec 18, 2018

Abstract

Sensitive detection of carcinoembryonic antigen (CEA) is very important for early detection and cancer therapy monitoring. We utilized the highly catalytic activity of MoS-gold nanoparticles (MoS-AuNPs) nanocomposites to construct a colorimetric immunoassay for CEA detection, which could catalyze sodium borohydride (NaBH) to reduce 4-nitrophenol (4-NP) and make the yellow solution colorless. MoS-AuNPs nanocomposites could efficiently load antibodies (Ab1 and Ab2) because of their good biocompatibility and high adsorption ability, forming Ab1-MoS-AuNPs nanoprobes for capturing CEA and Ab2-MoS-AuNPs nanoprobes for catalytic reaction. It should be pointed out that Ab2-MoS-AuNPs nanoprobes retained high catalytic activity because no blocking agent was used to block the catalytic sites. After the formation of the classical "sandwich" structure, parts of Ab2-MoS-AuNPs nanoprobes remained in the supernatant after centrifugation, making the catalytic reaction occur and obtaining a colorless solution. As expected, a linear function between absorbance peak intensity and the logarithm of 5 pg/mL-10 ng/mL CEA was observed, and the detection limit was obtained as 0.5 pg/mL. This colorimetric immunosensor showed acceptable selectivity and repeatability for CEA detection, which could be successfully employed to detect CEA in serum.

Authors

  • Shao Su
    Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
  • Jing Li
    Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.
  • Yao Yao
    Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
  • Qian Sun
    Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
  • Qiang Zhao
    Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
  • Fei Wang
    Department of Population Health Sciences, Weill Cornell Medicine, Cornell University, New York, NY, United States.
  • Qian Li
    Emergency and Critical Care Center, Department of Emergency Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
  • Xiaoguo Liu
    School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Lianhui Wang
    Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

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