Impact of Graphene Exposure on Microbial Activity and Community Ecosystem in Saliva.

Journal: ACS applied bio materials
Published Date: Dec 19, 2018

Abstract

Graphene-based nanomaterials (GMs) are served as great promising agents for the prevention and therapy of infectious diseases. However, their dental applications remain to be evaluated, especially under the context of the oral microbial community. Here, we examined the exposure-response of salivary bacterial community to two types of GMs, that is, graphene oxide (GO) and GO-silver nanoparticles (AgNPs). Both GO and GO-AgNPs showed lethal effect against salivary bacteria in a concentration-dependent manner, and the antibacterial capacity of GO-AgNPs is superior to GO. Interestingly, the salivary bacterial community enhanced the tolerance to GMs as compared to homogeneous bacteria. High-throughput sequencing revealed that both 80 μg/mL GO and 20 μg/mL GO-AgNPs significantly altered the biodiversity of salivary bacterial community. Especially, they increased the relative abundance of Gram-positive bacteria compared to the untreated sample, notably , suggesting that the bacterial wall structure plays a critical role in resisting the damage of GMs. Although GMs could effectively limit the salivary bacterial activity and cause changes in bacterial community structure, they are not toxic to mammalian cell lines. We envision this study could provide novel insights into the application of GMs as "green antibiotics" in nanomedicine.

Authors

  • Yuting Shi
    National Clinical Research Center of Oral Diseases, Shanghai 200011, China.
  • Wenjun Xia
    National Clinical Research Center of Oral Diseases, Shanghai 200011, China.
  • Shima Liu
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Jingyang Guo
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Zhengnan Qi
    National Clinical Research Center of Oral Diseases, Shanghai 200011, China.
  • Yan Zou
    National Clinical Research Center of Oral Diseases, Shanghai 200011, China.
  • Liping Wang
    School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200011, China.
  • Sheng-Zhong Duan
    National Clinical Research Center of Oral Diseases, Shanghai 200011, China.
  • Yi Zhou
    Eye Center of Xiangya Hospital, Central South University, Changsha, Hunan, China.
  • Chenglie Lin
    School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
  • Jiye Shi
    UCB Pharma, Slough, Berkshire SL1 3WE, U.K.
  • Lihua Wang
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Chunhai Fan
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Min Lv
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Zisheng Tang
    National Clinical Research Center of Oral Diseases, Shanghai 200011, China.

Keywords

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