Comparative extraction and simple isolation improvement techniques of active constituents' momilactone A and B from rice husks of by HPLC analysis and column chromatography.
Journal:
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society
Published Date:
Jul 20, 2018
Abstract
This paper reports comparative extraction efficiencies and enhancement methods for natural herbicidal (growth inhibitors) compounds, momilactone A and B, respectively from the dried husks of using different extraction techniques and different solvent systems. Four different extraction techniques . percolation, agitation with heat, sonication and soxhlet using five solvent systems as ethyl acetate, acetone, acetonitrile, methanol and methanol:water (8:2) were evaluated. In these studies, it was observed that maximum extract yield was obtained using in methanol and methanol/water mixture as extracting solvent by soxhlet technique although the content of total momilactones A and B was higher in the methanol/water mixture in comparison to other extractions. The successive and simple isolation enrichment technique for momilactones A and B were achieved by solid-matrix partitioning after the treatment of methanolic extract with charcoal and using ethyl acetate as extracting solvent for momilactones A and B. The quantitative analysis of the extraction and enrichment development protocol was validated by a simple, accurate, reproducible RP-HPLC-UV-VIS method using a binary gradient elution comprising of acetonitrile and water (70:30). The separation was achieved on a waters Spherisorb S10 ODS 2 column (250 × 4.6 mm, I.D., 10 µm) that achieved a greater degree of linearity within an overall concentration of extracts and momilactones A and B, 1 mg mL and higher degree of correlation (0.9928 ≤ r ≤ 0.9936) for momilactones A and B. So far, comparative extraction of momilactones A and B and HPLC of these compounds has not been reported. Standards of momilactones A () and B () were isolated along with other two compounds as orizaterpenoid () and 7-ketostigmaterol () from ethyl acetate extract of rice hulls of and checked purity by HPLC-PDA-MS and identification of these isolated compounds (-) by complete spectroscopic techniques as IR, H NMR, C NMR, 2D NMR and HR-MS. The qualitative analysis of momilactone A and B separation technique by thin layer chromatography was also developed.
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