Application of stabilizer improves stability of nanosuspended branched-chain amino acids and anti-inflammatory effect in LPS-induced RAW 264.7 cells.

Journal: Food science and biotechnology
Published Date: Dec 12, 2017

Abstract

This study investigated the use of polyglyceryl esters (PGE) as stabilizer in improving stability and anti-inflammatory activity of nanosuspended branched-chain amino acids (BCAAs). BCAAs nanosuspended with stabilizer (BS) exhibited improved stability at concentration of 5% saturation level during storage as compared to BCAAs nanosuspended with aqueous solution (BA). Additionally, anti-inflammatory activity of BS was found to be greater than that of BA. Nitric oxide scavenging activity was found to be dose-dependent, with activity of BS in sodium nitroprusside system being significantly higher than that of BA ( < 0.05) at 2.5-20 mg/mL. BS also possesses greater inhibitory activity on production of pro-inflammatory factors including inducible nitric oxide synthase, cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells through suppressed phosphorylation of p65 subunit of NF-κB at 0.5, 2, 8 mg/mL. These results suggest that PGE used as stabilizer improves solubility and biological activity of nanosuspended BCAAs.

Authors

  • Hyun Suk Kim
    1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Korea.
  • Hyung-Seok Yu
    1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Republic of Korea.
  • Jae Hoon Lee
    Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea.
  • Gyu Whan Lee
    R&D Center, Daesang Corporation, Icheon, Gyoenggi-do 17384 Korea.
  • Seung Jun Choi
    3Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
  • Pahn-Shick Chang
    4Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826 Korea.
  • Hyun-Dong Paik
    1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Republic of Korea.

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