Applications of random forest feature selection for fine-scale genetic population assignment.

Journal: Evolutionary applications
Published Date:

Abstract

Genetic population assignment used to inform wildlife management and conservation efforts requires panels of highly informative genetic markers and sensitive assignment tests. We explored the utility of machine-learning algorithms (random forest, regularized random forest and guided regularized random forest) compared with ranking for selection of single nucleotide polymorphisms (SNP) for fine-scale population assignment. We applied these methods to an unpublished SNP data set for Atlantic salmon () and a published SNP data set for Alaskan Chinook salmon (). In each species, we identified the minimum panel size required to obtain a self-assignment accuracy of at least 90% using each method to create panels of 50-700 markers Panels of SNPs identified using random forest-based methods performed up to 7.8 and 11.2 percentage points better than -selected panels of similar size for the Atlantic salmon and Chinook salmon data, respectively. Self-assignment accuracy ≥90% was obtained with panels of 670 and 384 SNPs for each data set, respectively, a level of accuracy never reached for these species using -selected panels. Our results demonstrate a role for machine-learning approaches in marker selection across large genomic data sets to improve assignment for management and conservation of exploited populations.

Authors

  • Emma V A Sylvester
    Faculty of Computer Science Dalhousie University Halifax NS Canada.
  • Paul Bentzen
    Marine Gene Probe Laboratory Department of Biology Dalhousie University Halifax NS Canada.
  • Ian R Bradbury
    Department of Fisheries and Oceans St. John's NL Canada.
  • Marie Clément
    Centre for Fisheries Ecosystems Research, Fisheries and Marine Institute Memorial University of Newfoundland St. John's NL Canada.
  • Jon Pearce
    Northern SE Regional Aquaculture Association Hidden Falls Hatchery Sitka AK USA.
  • John Horne
    Marine Gene Probe Laboratory Department of Biology Dalhousie University Halifax NS Canada.
  • Robert G Beiko
    Faculty of Computer Science Dalhousie University Halifax NS Canada.

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