Phytochemicals from Chi Flowers Reduce the Pyocyanin Production and Motility of PAO1.

Journal: Frontiers in microbiology
Published Date: Jan 9, 2018

Abstract

Chi, known as a medicinal and edible plant in China, exhibits multiple bioactivities, especially antibacterial activity. In this study, we investigated the inhibitory effects of the dichloromethane fraction (DF) of Chi flowers on the pyocyanin production, swarming motility, and swimming motility of PAO1, at sub-minimum inhibitory concentrations. Results showed that the DF had a remarkable inhibitory effect on pyocyanin production without influencing PAO1 growth, and concentration-dependent inhibitory effects on swarming and swimming motility. The half maximal inhibitory concentrations (IC) were 0.158 ± 0.009, 0.139 ± 0.004, and 0.334 ± 0.049 mg/mL for pyocyanin production, swarming motility, and swimming motility, respectively. Real-time RT-PCR showed that the DF significantly down-regulated the expressions of ( < 0.05) and ( < 0.01). In addition, gallic acid, catechin, ellagic acid, chlorogenic acid, quercetin, and kaempferol were identified in the DF by HPLC Triple TOF MS/MS analysis. All six identified compounds showed inhibitory effects on pyocyanin production, swarming motility, and swimming motility, though ellagic acid showed the strongest effects, with IC values of 0.067 ± 0.002, 0.024 ± 0.008, and 0.020 ± 0.003 mg/mL, respectively. Thus, the inhibitory effects on PAO1 virulence factors might be attributable to these six and/or other compounds in the DF of Chi flowers. Consequently, the Chi flower, especially the DF, might be a potential quorum sensing inhibitor of PAO1.

Authors

  • Rui Yang
    Department of Biomedical Informatics, Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore.
  • Ying Guan
    Inspection and Pattern Evaluation Department, Suzhou Institute of Measurement and Testing, Suzhou, China.
  • Jinwei Zhou
    School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, China.
  • Bing Sun
    School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, China.
  • Zhennan Wang
    School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, China.
  • Hongjuan Chen
    State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
  • Zhaochun He
    School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, China.
  • Aiqun Jia
    School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, China.

Keywords

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