Examination of Quaternary Ammonium Compound Resistance in Isolated from Cooked Meat Products in China.
Journal:
Frontiers in microbiology
Published Date:
Dec 11, 2017
Abstract
The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of genes with class 1 integrons in isolated from cooked meat products. A total of 52 isolates (29.2%) were detected from 178 samples, and their minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to >32 μg/mL. The isolates with BC MICs of 24 μg/mL were observed most frequently. PCR assays indicated that /Δ1, , and were commonly present (32.7%-100%) in these isolates, but was less prevalent (3.8%). Five groups of resistance gene cassettes were identified in 10 -positive isolates. An unusual gene cassette array -- was found in one foodborne isolate of . Two isolates harbored - and - associated non-classic integrons: IS and a new arrangement --IS, which is first reported in . Non-classic class 1 integrons were located on conjugative plasmids of 100 kb in two tested isolates. Our data showed that the QAC resistance genes were commonly present among isolates from cooked meats and was associated with non-classic class 1 integrons. The creation of transconjugants demonstrated that -associated non-classic class 1 integrons were located on conjugative plasmids and therefore could facilitate the co-dissemination of disinfectant and antimicrobial resistance genes among bacteria, an increasing area of concern.
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