Antibacterial and Antioxidant Activities of Novel Strain Isolated from Gulf of Khambhat, Gujarat.
Journal:
Frontiers in microbiology
Published Date:
Dec 7, 2017
Abstract
Bacterial secondary metabolites possess a wide range of biologically active compounds including antibacterial and antioxidants. In this study, a Gram-positive novel marine was isolated from sea sediment which showed 84% 16S rRNA gene sequence (KT588655) similarity with (EU841661) and designated as RD-5. The genus is considered as a promising source of bioactive secondary metabolites. The isolated novel bacterial strain was characterized by antibacterial characteristics and antioxidant activities. The BIOLOG based analysis suggested that RD-5 utilized a wide range of substrates compared to the reference strain. The result is further supported by statistical analysis such as AWCD (average well color development), heat-map and PCA (principal component analysis). The whole cell fatty acid profiling showed the dominance of iso/anteiso branched C15-C17 long chain fatty acids. The identified strain RD-5 exhibited a broad spectrum of antibacterial activities for the Gram-negative bacteria ( NCIM 2065, NCIM, sp. NCIM 2200 and NCIM), and Gram-positive bacteria ( NCIM 2920 and MTCC 96). Extract of strain RD-5 showed 82.86 and 89% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and metal chelating activity, respectively, at 5.0 mg/mL. While HO scavenging activity was 74.5% at 0.05 mg/mL concentration. Furthermore, polyketide synthases (PKSs types I and II), an enzyme complex that produces polyketides, the encoding gene(s) detected in the strain RD-5 which may probably involve for the synthesis of antibacterial compound(s). In conclusion, a novel bacterial strain of , isolated from the unexplored sea sediment of Alang, Gulf of Khambhat (Gujarat), India showed promising antibacterial activities. However, fractionation and further characterization of active compounds from RD-5 are needed for their optimum utilization toward antibacterial purposes.
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