Evidence for Anti-Pseudogymnoascus destructans (Pd) Activity of Propolis.

Journal: Antibiotics (Basel, Switzerland)
Published Date: Dec 21, 2017

Abstract

White-nose syndrome (WNS) in bats, caused by (), is a cutaneous infection that has devastated North American bat populations since 2007. At present, there is no effective method for controlling this disease. Here, we evaluated the effect of propolis against in vitro. Using Sabouraud dextrose agar (SDA) medium, approximately 1.7 × 10⁷ conidia spores of the strain M3906-2/mL were spread on each plate and grown to form a consistent lawn. A Kirby-Bauer disk diffusion assay was employed using different concentrations of propolis (1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%), in plates incubated at 8 °C and 15 °C. At 8 °C and 15 °C, as the concentration of propolis increased, there was an increasing zone of inhibition (ZOI), reaching the highest degree at 10% and 25% concentrations, respectively. A germule suppression assay showed a similar effect on conidia germination. A MALDI-TOF-MS analysis of propolis revealed multiple constituents with a potential anti- activity, including cinnamic acid, p-coumaric acid, and dihydrochalcones, which could be further tested for their individual effects. Our study suggests that propolis or its individual constituents might be suitable products against .

Authors

  • Soumya Ghosh
    Department of Biological Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada. sghosh@tru.ca.
  • Robyn McArthur
    Department of Biological Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada. rmcarthur645@gmail.com.
  • Zhi Chao Guo
    Department of Biological Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada. michelle8472@hotmail.com.
  • Rory McKerchar
    Department of Biological Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada. rory-mckerchar@hotmail.com.
  • Kingsley Donkor
    Department of Biological Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada. kdonkor@tru.ca.
  • Jianping Xu
    Department of Biology, McMaster University, Hamilton, ON L8S 4K1 Canada. jpxu@mcmaster.ca.
  • Naowarat Cheeptham
    Department of Biological Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada. ncheeptham@tru.ca.

Keywords

No keywords available for this article.

External Resources

Popular Topics
Recent Journals