Inhibition of EGF expression and NF-κB activity by treatment with quercetin leads to suppression of angiogenesis in nasopharyngeal carcinoma.

Journal: Saudi journal of biological sciences
Published Date: Nov 21, 2016

Abstract

The present study was performed to investigate the effect of quercetin on nasopharyngeal carcinoma (NPC) angiogenesis. The real-time RT-PCR and enzyme-linked immunosorbent assays (ELISA) were performed to analyze the expression levels of vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma cell lines prior to and after the quercetin treatment. Effect of quercetin on the rate of cell proliferation was measured by MTT assay. It was observed that quercetin treatment at a concentration of 10 mg/mL reduced the rate of NPC039 cell viability to 36% compared to control after 24 h. The expression of VEGF and activity of NF-κB was also markedly reduced. The ability of tube formation in HUVECs was inhibited significantly on exposure to quercetin compared to the untreated cells. Therefore, quercetin plays an important role in the inhibition of NPC039 nasopharyngeal carcinoma and can be of therapeutic importance.

Authors

  • Dong-Yan Huang
    Department of Otolaryngology-Head and Neck Surgery, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing 100853, China.
  • Zhi-Rao Dai
    Department of Otolaryngology, The First Affiliated Hospital of PLA General Hospital, Beijing 100853, China.
  • Wei-Min Li
    Department of Otolaryngology-Head and Neck Surgery, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing 100853, China.
  • Rong-Guan Wang
    Department of Otolaryngology-Head and Neck Surgery, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing 100853, China.
  • Shi-Ming Yang
    Department of Otolaryngology-Head and Neck Surgery, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing 100853, China.

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