In vitro ovicidal activity of seeds extract on the eggs of .
Journal:
Journal of parasitic diseases : official organ of the Indian Society for Parasitology
Published Date:
Aug 29, 2016
Abstract
seeds extract has been previously reported to have antimicrobial and other medicinal properties. The aim of this study was to evaluate the ovicidal activity of the methanolic extract of seeds against the eggs of The phenolic compounds of the methanolic extract of seeds were identified by HPLC analysis. Catechin, rutin, p-Coumaric acid, chloregenic acid and hesperetin were found to be the major phenolic compounds. eggs were collected from the gall bladder of naturally infected sheep. The eggs were exposed to two concentrations of seeds extract (1 and 3 mg/mL) for 24 and 48 h. To investigate the effect of the seeds extract on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that eggs were susceptible to the methanolic extract of seeds. Following 24 h exposure of the eggs to seeds extract with concentrations of 1 and 3 mg/mL, the miracidial formation reduced to 5 and 2.2 % respectively (compared with 60 % for the control group). Following 48 h of exposure of the eggs to seeds extract with 1 mg/mL concentration, the miracidial formation reduced to 0.5 %. In this exposure time, no miracidial formation was observed in the eggs exposed to seeds extract with concentration of 3 mg/mL. Therefore, the results of this study indicated that seeds extract has high ovicidal activity against the eggs of . Accordingly, this extract may have the potential flukicidal activity against the immature and mature .
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