Simple and Versatile Detection of Viruses Using Anodized Alumina Membranes.

Journal: ACS sensors
Published Date: Mar 8, 2016

Abstract

A simple sensor for viral particles based on ionic conductivity through anodized alumina membranes was demonstrated using MS2 bacteriophage as an example. A facile two-point measuring scheme is geared toward realization using a computer's sound card input/output capabilities suitable for a fast and inexpensive point of care testing. The lowest detection concentration down to ~7 pfu/mL and a large dynamic range up to ~2000 pfu/mL were obtained due to physical optimization that included proper length and diameter for the pores, removing the oxide layer at the electrode, as well as the chemical optimization of covalent binding of antibodies to the pore's walls.

Authors

  • Pavan Chaturvedi
    Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, United States.
  • Stacy D Rodriguez
    Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003, United States.
  • Ivan Vlassiouk
    Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.
  • Immo A Hansen
    Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003, United States.
  • Sergei N Smirnov
    Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, United States.

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