visualization and quantification of experimental myocardial infarction by indocyanine green fluorescence imaging.

Journal: Biomedical optics express
Published Date: Dec 8, 2016

Abstract

The fluorophore indocyanine green accumulates in areas of ischemia-reperfusion injury due to an increase in vascular permeability and extravasation of the dye. The aim of the study was to validate an indocyanine green-based technique of in vivo visualization of myocardial infarction. A further aim was to quantify infarct size ex vivo and compare this technique with the standard triphenyltetrazolium chloride staining. Wistar rats were subjected to regional myocardial ischemia (30 minutes) followed by reperfusion (n = 7). Indocyanine green (0.25 mg/mL in 1 mL of normal saline) was infused intravenously for 10 minutes starting from the 25th minute of ischemia. Video registration in the near-infrared fluorescence was performed. Epicardial fluorescence of indocyanine green corresponded to the injured area after 30 minutes of reperfusion. Infarct size was similar when determined ex vivo using traditional triphenyltetrazolium chloride assay and indocyanine green fluorescent labeling. Intravital visualization of irreversible injury can be done directly by fluorescence on the surface of the heart. This technique may also be an alternative for ex vivo measurements of infarct size.

Authors

  • Dmitry Sonin
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia; Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia; ITMO University, Kronverksky Avenue 49, 197101 St. Petersburg, Russia.
  • Garry Papayan
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia; Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia; ITMO University, Kronverksky Avenue 49, 197101 St. Petersburg, Russia.
  • Evgeniia Pochkaeva
    Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia.
  • Svetlana Chefu
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia; Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia.
  • Sarkis Minasian
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia; Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia; ITMO University, Kronverksky Avenue 49, 197101 St. Petersburg, Russia.
  • Dmitry Kurapeev
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia.
  • Jarle Vaage
    ITMO University, Kronverksky Avenue 49, 197101 St. Petersburg, Russia; Institute of Clinical Medicine, University of Oslo and Department of Emergency and Intensive Care Medicine, Oslo University Hospital, Postboks 1171, Blindern, 0318 Oslo, Norway.
  • Nickolay Petrishchev
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia; Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia; ITMO University, Kronverksky Avenue 49, 197101 St. Petersburg, Russia.
  • Michael Galagudza
    Center for Laser Medicine, Department of Pathophysiology, First I.P. Pavlov Federal Medical University of St. Petersburg, Lev Tolstoy Str. 6/8, 197022, St. Petersburg, Russia; Institute of Experimental Medicine, Federal Almazov Medical Research Centre, Akkuratova Str. 2, 197341, St. Petersburg, Russia; ITMO University, Kronverksky Avenue 49, 197101 St. Petersburg, Russia.

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