Human heart failure biomarker immunosensor based on excessively tilted fiber gratings.

Journal: Biomedical optics express
Published Date: Dec 5, 2016

Abstract

A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NT-proBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.

Authors

  • Binbin Luo
    Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China; School of Opto-electronic Information, University of Electronic Science and Technology of China, Chengdu, 610054, China; Aston Institute of Photonic Technologies, Aston University, Birmingham, B4 7ET, UK; luobinbin@cqut.edu.cn.
  • Shengxi Wu
    School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.
  • Zhonghao Zhang
    School of Information and Communication EngineeringUniversity of Electronic Science and Technology of China (UESTC)Chengdu611731China.
  • Wengen Zou
    Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
  • Shenghui Shi
    Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
  • Mingfu Zhao
    Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
  • Nianbing Zhong
    Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
  • Yong Liu
    Department of Critical care medicine, Shenzhen Hospital, Southern Medical University, Guangdong, Shenzhen, China.
  • Xue Zou
    Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
  • Lingling Wang
    Department of Neurology, Shanghai Pudong New Area People's Hospital, Shanghai, China.
  • Weina Chai
    The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400050, China.
  • Chuanmin Hu
    Department of Clinical Biochemistry, Third Military Medical University, Chongqing, 400038, China.
  • Lin Zhang
    Laboratory of Molecular Translational Medicine, Centre for Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Clinical Research Center for Birth Defects of Sichuan Province, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. Electronic address: zhanglin@scu.edu.cn.

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