Multiple Cross Displacement Amplification Combined with Gold Nanoparticle-Based Lateral Flow Biosensor for Detection of .

Journal: Frontiers in microbiology
Published Date: Dec 22, 2016

Abstract

() is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. In the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (MCDA-LFB) to detect the gene of in DNA extracts from pure cultures and spiked oyster homogenates. Amplification was carried out at a constant temperature (62°C) for only 30 min, and amplification products were directly applied to the biosensor. The entire process, including oyster homogenate processing (30 min), isothermal amplification (30 min) and results indicating (∼2 min), could be completed within 65 min. Amplification product was detectable from as little as 10 fg of pure DNA and from approximately 4.2 × 10 CFU in 1 mL of oyster homogenate. No cross-reaction with other species and with non- species was observed. Therefore, the MCDA-LFB method established in the current report is suitable for the rapid screening of in clinical, food, and environmental samples.

Authors

  • Yi Wang
    Department of Neurology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China.
  • Hui Li
    Department of Ophthalmology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.
  • Dongxun Li
    Changping District Center for Disease Control and Prevention Beijing, China.
  • Kewei Li
    Institute of Microbiology, Jilin Provincial Center for Disease Control and Prevention Changchun, China.
  • Yan Wang
    College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
  • Jianguo Xu
  • Changyun Ye
    State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention Beijing, China.

Keywords

No keywords available for this article.

External Resources

Popular Topics
Recent Journals