AIMC Journal:
Biotechnic & histochemistry : official publication of the Biological Stain Commission
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Comparison of manual counting with two rule-based image analysis programs, ImageJ and CellProfiler, for quantitative analysis of c-Fos immunohistochemical positivity in rat brainstem sections.
Biotechnic & histochemistry : official publication of the Biological Stain Commission
Sep 17, 2026
Quantitative and semiquantitative assessment of biomarker expression using immunohistochemistry (IHC) can be performed using several analytical approaches. Manual counting remains widely used but is inherently subjective and susceptible to observer v...
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