The IBEX Knowledge-Base: A central resource for multiplexed imaging techniques.

Journal: PLoS biology
PMID:

Abstract

Multiplexed imaging is a powerful approach in spatial biology, although it is complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, a central resource for reagents, protocols and more, to enhance knowledge sharing, optimization and innovation of spatial proteomics techniques.

Authors

  • Andrea J Radtke
    Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Ifeanyichukwu U Anidi
    Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Leanne Arakkal
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Armando J Arroyo-Mejias
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Rebecca T Beuschel
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Katy Börner
    Luddy School of Informatics, Computing, and Engineering, Indiana University, Bloomington, IN, United States of America.
  • Colin J Chu
    UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre, London, United Kingdom.
  • Beatrice Clark
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Menna R Clatworthy
    Molecular Immunity Unit, Laboratory of Molecular Biology, Cambridge Institute for Therapeutic Immunology and Infectious Diseases, University of Cambridge Department of Medicine, Cambridge, United Kingdom.
  • Jake Colautti
    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada.
  • Fabian Coscia
    NNF Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Joshua Croteau
    Department of Business Development, BioLegend Inc., San Diego, California, United States of America.
  • Saven Denha
    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada.
  • Rose Dever
    Functional Immunogenomics Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Walderez O Dutra
    Laboratory of Cell-Cell Interactions, Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Sonja Fritzsche
    Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany.
  • Spencer Fullam
    Division of Rheumatology, Rush University Medical Center, Chicago, Illinois, United States of America.
  • Michael Y Gerner
    Department of Immunology, University of Washington School of Medicine, Seattle, Washington, United States of America.
  • Anita Gola
    Robin Chemers Neustein Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York, United States of America.
  • Kenneth J Gollob
    Center for Research in Immuno-oncology (CRIO), Hospital Israelita Albert Einstein, São Paulo, Brazil.
  • Jonathan M Hernandez
    Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Jyh Liang Hor
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Hiroshi Ichise
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Zhixin Jing
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Danny Jonigk
    Institute of Pathology, Hannover Medical School, and the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 30625, Hanover, Germany.
  • Evelyn Kandov
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Wolfgang Kastenmüller
    Würzburg Institute of Systems Immunology, Max Planck Research Group at the Julius-Maximilians-Universität Würzburg, Würzburg, Germany.
  • Joshua F E Koenig
    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada.
  • Rosa K Kortekaas
    Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph's Healthcare, Hamilton, Canada.
  • Aanandita Kothurkar
    UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre, London, United Kingdom.
  • Alexandra Y Kreins
    Infection Immunity and Inflammation Research and Teaching Department, University College London Great Ormond Street Institute of Child Health, London, United Kingdom.
  • Ian T Lamborn
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Yuri Lin
    Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Katia Luciano Pereira Morais
    Center for Research in Immuno-oncology (CRIO), Hospital Israelita Albert Einstein, São Paulo, Brazil.
  • Aleksandra Lunich
    Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Jean C S Luz
    Viral Vector Laboratory, Cancer Institute of São Paulo, University of São Paulo, São Paulo, Brazil.
  • Ryan B MacDonald
    UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre, London, United Kingdom.
  • Chen Makranz
    Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Vivien I Maltez
    Division of Allergy, Immunology and Rheumatology, Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America.
  • John E McDonough
    Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph's Healthcare, Hamilton, Canada.
  • Ryan V Moriarty
    Department of Cellular and Developmental Biology, Northwestern University, Chicago, Illinois, United States of America.
  • Juan M Ocampo-Godinez
    Infection Immunity and Inflammation Research and Teaching Department, University College London Great Ormond Street Institute of Child Health, London, United Kingdom.
  • Vitoria M Olyntho
    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada.
  • Annette Oxenius
    Institute of Microbiology, ETH Zürich, Vladimir-Prelog-Weg 4, 8093 Zürich, Switzerland. Electronic address: aoxenius@micro.biol.ethz.ch.
  • Kartika Padhan
    Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Kirsten Remmert
    Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Nathan Richoz
    Molecular Immunity Unit, Laboratory of Molecular Biology, Cambridge Institute for Therapeutic Immunology and Infectious Diseases, University of Cambridge Department of Medicine, Cambridge, United Kingdom.
  • Edward C Schrom
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Wanjing Shang
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Lihong Shi
    College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China. Electronic address: shilihong@sxu.edu.cn.
  • Rochelle M Shih
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Emily Speranza
    Florida Research and Innovation Center, Cleveland Clinic Lerner Research Institute, Port Saint Lucie, Florida, United States of America.
  • Salome Stierli
    Institute of Anatomy, University of Zurich, Zurich, Switzerland.
  • Sarah A Teichmann
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
  • Tibor Z Veres
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Megan Vierhout
    Department of Medicine, McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Canada.
  • Brianna T Wachter
    Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Adam K Wade-Vallance
    Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Margaret Williams
    Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
  • Nathan Zangger
    Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
  • Ronald N Germain
  • Ziv Yaniv
    MSC LLC., Rockville, MD, 20852, USA.