DRAG: design RNAs as hierarchical graphs with reinforcement learning.

Journal: Briefings in bioinformatics
PMID:

Abstract

The rapid development of RNA vaccines and therapeutics puts forward intensive requirements on the sequence design of RNAs. RNA sequence design, or RNA inverse folding, aims to generate RNA sequences that can fold into specific target structures. To date, efficient and high-accuracy prediction models for secondary structures of RNAs have been developed. They provide a basis for computational RNA sequence design methods. Especially, reinforcement learning (RL) has emerged as a promising approach for RNA design due to its ability to learn from trial and error in generation tasks and work without ground truth data. However, existing RL methods are limited in considering complex hierarchical structures in RNA design environments. To address the above limitation, we propose DRAG, an RL method that builds design environments for target secondary structures with hierarchical division based on graph neural networks. Through extensive experiments on benchmark datasets, DRAG exhibits remarkable performance compared with current machine-learning approaches for RNA sequence design. This advantage is particularly evident in long and intricate tasks involving structures with significant depth.

Authors

  • Yichong Li
    Department of Computer Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Rd., Minhang District, Shanghai 200240, China.
  • Xiaoyong Pan
    Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, Denmark. xypan172436@gmail.com.
  • Hongbin Shen
  • Yang Yang
    Department of Gastrointestinal Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, China.