Enabling malic acid production from corn-stover hydrolysate in Lipomyces starkeyi via metabolic engineering and bioprocess optimization.

Journal: Microbial cell factories
Published Date: May 21, 2025

Abstract

BACKGROUND: Lipomyces starkeyi is an oleaginous yeast with a native metabolism well-suited for production of lipids and biofuels from complex lignocellulosic and waste feedstocks. Recent advances in genetic engineering tools have facilitated the development of L. starkeyi into a microbial chassis for biofuel and chemical production. However, the feasibility of redirecting L. starkeyi lipid flux away from lipids and towards other products remains relatively unexplored. Here, we engineer the native metabolism to produce malic acid by introducing the reductive TCA pathway and a C4-dicarboxylic acid transporter to the yeast.

Authors

  • Jeffrey J Czajka
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Ziyu Dai
    Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
  • Tijana Radivojević
    DOE Agile BioFoundry, Emeryville, CA, 94608, USA.
  • Joonhoon Kim
    Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Pacific Northwest National Laboratory, Richland, 99354, WA, USA.
  • Shuang Deng
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Teresa Lemmon
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Marie Swita
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Meagan C Burnet
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
  • Nathalie Munoz
    DOE Agile BioFoundry, Emeryville, CA, 94608, USA.
  • Yuqian Gao
    DOE Agile BioFoundry, Emeryville, CA, 94608, USA.
  • Young-Mo Kim
    DOE Agile BioFoundry, Emeryville, CA, 94608, USA.
  • Beth Hofstad
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Jon K Magnuson
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Hector Garcia Martin
    Joint BioEnergy Institute (JBEI) , Emeryville , California 94608 , United States.
  • Kristin E Burnum-Johnson
    DOE Agile BioFoundry, Emeryville, CA, 94608, USA.
  • Kyle R Pomraning
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA. kyle.pomraning@pnnl.gov.

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