Iterative unsupervised domain adaptation for generalized cell detection from brightfield z-stacks.

Journal: BMC bioinformatics
Published Date:

Abstract

BACKGROUND: Cell counting from cell cultures is required in multiple biological and biomedical research applications. Especially, accurate brightfield-based cell counting methods are needed for cell growth analysis. With deep learning, cells can be detected with high accuracy, but manually annotated training data is required. We propose a method for cell detection that requires annotated training data for one cell line only, and generalizes to other, unseen cell lines.

Authors

  • Kaisa Liimatainen
    BioMediTech and Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.
  • Lauri Kananen
    Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
  • Leena Latonen
    BioMediTech and Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.
  • Pekka Ruusuvuori
    BioMediTech and Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.