Performing protein fold recognition by exploiting a stack convolutional neural network with the attention mechanism.

Journal: Analytical biochemistry
Published Date:

Abstract

Protein fold recognition is a critical step in protein structure and function prediction, and aims to ascertain the most likely fold type of the query protein. As a typical pattern recognition problem, designing a powerful feature extractor and metric function to extract relevant and representative fold-specific features from protein sequences is the key to improving protein fold recognition. In this study, we propose an effective sequence-based approach, called RattnetFold, to identify protein fold types. The basic concept of RattnetFold is to employ a stack convolutional neural network with the attention mechanism that acts as a feature extractor to extract fold-specific features from protein residue-residue contact maps. Moreover, based on the fold-specific features, we leverage metric learning to project fold-specific features into a subspace where similar proteins are closer together and name this approach RattnetFoldPro. Benchmarking experiments illustrate that RattnetFold and RattnetFoldPro enable the convolutional neural networks to efficiently learn the underlying subtle patterns in residue-residue contact maps, thereby improving the performance of protein fold recognition. An online web server of RattnetFold and the benchmark datasets are freely available at http://csbio.njust.edu.cn/bioinf/rattnetfold/.

Authors

  • Ke Han
    School of Computer and Information Engineering, Harbin University of Commerce, Harbin 150040, China. hanke@hrbcu.edu.cn.
  • Yan Liu
    Department of Clinical Microbiology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, People's Republic of China.
  • Jian Xu
    Department of Cardiology, Lishui Central Hospital and the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.
  • Jiangning Song
    College of Information Engineering, Northwest A&F University, Yangling 712100, China, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia, National Engineering Laboratory for Industrial Enzymes and Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Centre for Research in Intelligent Systems, Faculty of Information Technology, Monash University, Melbourne, VIC 3800, Australia and ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, VIC 3800, Australia College of Information Engineering, Northwest A&F University, Yangling 712100, China, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia, National Engineering Laboratory for Industrial Enzymes and Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Centre for Research in Intelligent Systems, Faculty of Information Technology, Monash University, Melbourne, VIC 3800, Australia and ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, VIC 3800, Australia College of Information Engineering, Northwest A&F University, Yangling 712100, China, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia, National Engineering Laboratory for Industrial Enzymes and Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, Centre for Research in Intelligent Systems, Faculty of Information Technology, Monash University, Melbourne, VIC 3800, Australia and ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, VIC 3800, Australia.
  • Dong-Jun Yu