[Frontiers and development in live-cell super-resolution fluorescence microscopy].

Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Published Date:

Abstract

This paper reviews the research progress on live-cell super-resolution fluorescence microscopy, discusses the current research status and hotspots in this field, and summarizes the technological application of super-resolution fluorescence microscopy for live-cell imaging. To date, this field has gained progress in numerous aspects. Specifically, the structured illumination microscopy, stimulated emission depletion microscopy, and the recently introduced minimal photon fluxes microscopy are the current research hotspots. According to the current progress in this field, future development trend is likely to be largely driven by artificial intelligence as well as advances in fluorescent probes and relevant labelling methods.

Authors

  • Yufei Cheng
    Core Facilities, West China Hospital of Sichuan University, Chengdu 610041, P. R. China.
  • Wei Li
    Department of Nephrology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
  • Tingting Jin
    Department of Health Policy and Management, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, P. R. China.
  • Sisi Wu
    Core Facilities, West China Hospital of Sichuan University, Chengdu 610041, P. R. China.
  • Longhao Zhang
    Department of Medical Discipline Construction, West China hospital of Sichuan University, Chengdu 610041, P. R. China.