Effect of bovine lactoferrin and human lactoferrin on the proliferative activity of the osteoblast cell line MC3T3-E1 in vitro.

Journal: Journal of dairy science
PMID: 29290425

Abstract

We conducted a comparative in vitro study on the proliferative effects of natural human lactoferrin (nhLF) and bovine lactoferrin (bLF) on osteoblasts. We investigated cell proliferation, cell survival, cell cycle, and mRNA and protein expression of proliferating cell nuclear antigen. Results indicated that treatment with 100 μg/mL of bLF or nhLF promoted the proliferation and sustenance of osteoblasts, and increased the length of the G/M and S phases compared with the untreated osteoblasts. Results of real-time quantitative PCR and Western blot showed that mRNA and protein expression of proliferating cell nuclear antigen by osteoblasts treated with bLF or nhLF were greater than those of the untreated control. At the same concentration, bLF demonstrated a greater effect on osteoblast proliferation than did nhLF. This study provides insights of significance in the utlization of bLF in healthy food formulas.

Authors

  • J L Zhang
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China.
  • X Han
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China. Electronic address: xhan@hit.edu.cn.
  • Y J Shan
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China.
  • L W Zhang
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China; College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China. Electronic address: zhanglw@hit.edu.cn.
  • M Du
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China.
  • M Liu
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China.
  • H X Yi
    College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
  • Y Ma
    School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, China.

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