Expression and Purification of SMGL-1.
Journal:
Studies in health technology and informatics
Published Date:
Nov 23, 2023
Abstract
Recombinant protein expression is a crucial technique in biology, with E. coli being the most widely used expression system. However, due to growth pressure, the expression of large molecular weight proteins in E. coli has remained a challenging task. SMGL-1, a newly discovered protein in C. elegans with Rabin 8 function, plays an important role in biology. To better understand the function of SMGL-1, we first predicted and analyzed its protein structure and properties using artificial intelligence. We then conducted studies on its expression and purification. Through optimization of IPTG concentration and expression strains, we successfully expressed SMGL-1 in E. coli, providing guidance for the expression of large proteins in E. coli. Furthermore, we explored the purification of SMGL-1 using GST affinity chromatography, Nickel affinity chromatography, and ammonium sulfate precipitation methods, laying the foundation for future purification work on SMGL-1.