Improving the enzymatic activity and stability of N-carbamoyl hydrolase using deep learning approach.

Journal: Microbial cell factories
PMID: 38834993

Abstract

BACKGROUND: Optically active D-amino acids are widely used as intermediates in the synthesis of antibiotics, insecticides, and peptide hormones. Currently, the two-enzyme cascade reaction is the most efficient way to produce D-amino acids using enzymes DHdt and DCase, but DCase is susceptible to heat inactivation. Here, to enhance the enzymatic activity and thermal stability of DCase, a rational design software "Feitian" was developed based on k prediction using the deep learning approach.

Authors

  • Fa Zhang
    High Performance Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, China.
  • Muhammad Naeem
    Department of Microbiology, Government College University, Faisalabad, Pakistan. muhammadnaeem@gcuf.edu.pk.
  • Bo Yu
    Department of Cardiology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Feixia Liu
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. liufeixia@im.ac.cn.
  • Jiansong Ju

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