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Aminohydrolases

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Machine learning modelling for the ultrasonication-mediated disruption of recombinant E. coli for the efficient release of nitrilase.

Ultrasonics
The ultrasonication-mediated cell disruption of recombinant E. coli was modeled using three machine learning techniques namely Multiple linear regression (MLR), Multi-layer perceptron (MLP) and Sequential minimal optimization (SMO). The four attribut...

Machine learning-based prediction of enzyme substrate scope: Application to bacterial nitrilases.

Proteins
Predicting the range of substrates accepted by an enzyme from its amino acid sequence is challenging. Although sequence- and structure-based annotation approaches are often accurate for predicting broad categories of substrate specificity, they gener...