Preventing illegal seafood trade using machine-learning assisted microbiome analysis.

Journal: BMC biology
PMID:

Abstract

BACKGROUND: Seafood is increasingly traded worldwide, but its supply chain is particularly prone to frauds. To increase consumer confidence, prevent illegal trade, and provide independent validation for eco-labelling, accurate tools for seafood traceability are needed. Here we show that the use of microbiome profiling (MP) coupled with machine learning (ML) allows precise tracing the origin of Manila clams harvested in areas separated by small geographic distances. The study was designed to represent a real-world scenario. Clams were collected in different seasons across the most important production area in Europe (lagoons along the northern Adriatic coast) to cover the known seasonal variation in microbiome composition for the species. DNA extracted from samples underwent the same depuration process as commercial products (i.e. at least 12 h in open flow systems).

Authors

  • Luca Peruzza
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.
  • Francesco Cicala
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.
  • Massimo Milan
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy. massimo.milan@unipd.it.
  • Giulia Dalla Rovere
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.
  • Tomaso Patarnello
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.
  • Luciano Boffo
    La Vongola Verace Di Chioggia, Chioggia, Italy.
  • Morgan Smits
    LEMAR, UMR 6539 CNRS/UBO/IRD/IFREMER, Institut Universitaire Européen de La Mer, Place Nicolas Copernic, Plouzané, 29280, France.
  • Silvia Iori
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.
  • Angelo De Bortoli
    Experteam S.R.L, Via Della Libertà, 12, Marghera, 30175, Italy.
  • Federica Schiavon
    Experteam S.R.L, Via Della Libertà, 12, Marghera, 30175, Italy.
  • Aurelio Zentilin
    Almar Soc. Coop. Agricola Arl, Via G. Raddi, 2, Marano Lagunare, 33050, Italy.
  • Piero Fariselli
  • Barbara Cardazzo
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.
  • Luca Bargelloni
    Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell'Università 16, Legnaro, 35020, Italy.