FlyVISTA, an integrated machine learning platform for deep phenotyping of sleep in .

Journal: Science advances
PMID: 40073129

Abstract

There is great interest in using genetically tractable organisms such as to gain insights into the regulation and function of sleep. However, sleep phenotyping in has largely relied on simple measures of locomotor inactivity. Here, we present FlyVISTA, a machine learning platform to perform deep phenotyping of sleep in flies. This platform comprises a high-resolution closed-loop video imaging system, coupled with a deep learning network to annotate 35 body parts, and a computational pipeline to extract behaviors from high-dimensional data. FlyVISTA reveals the distinct spatiotemporal dynamics of sleep and wake-associated microbehaviors at baseline, following administration of the sleep-inducing drug gaboxadol, and with dorsal fan-shaped body drivers. We identify a microbehavior ("haltere switch") exclusively seen during quiescence that indicates a deeper sleep stage. These results enable the rigorous analysis of sleep in and set the stage for computational analyses of microbehaviors in quiescent animals.

Authors

  • Mehmet F Keleş
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Ali Osman Berk Sapci
    Department of Computer Science, Sabanci University, Tuzla, Istanbul 34956, Turkey.
  • Casey Brody
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Isabelle Palmer
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Anuradha Mehta
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Shahin Ahmadi
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Christin Le
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Oznur Tastan
  • Sündüz Keleş
    Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI 53706, USA.
  • Mark N Wu
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.

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