Increase Docking Score Screening Power by Simple Fusion With CNNscore.

Journal: Journal of computational chemistry
PMID:

Abstract

Scoring functions (SFs) of molecular docking is a vital component of structure-based virtual screening (SBVS). Traditional SFs yield their inherent shortage for idealized approximations and simplifications predicting the binding affinity. Complementarily, SFs based on deep learning (DL) have emerged as powerful tools for capturing intricate features within protein-ligand (PL) interactions. We here present a docking-score fusion strategy that integrates pose scores derived from GNINA's convolutional neural network (CNN) with traditional docking scores. Extensive validation on diverse datasets has shown that by means of multiplying Watvina docking score by CNNscore demonstrates state-of-the-art screening power. Furthermore, in a reverse practice, our docking-score fusion technique was incorporated into the virtual screening (VS) workflow aimed at identifying inhibitors of the challenging target TYK2. Two promising hits with IC 9.99 μM and 13.76 μM in vitro were identified from nearly 12 billion molecules.

Authors

  • Huicong Liang
    Marine Biomedical Research Institute of Qingdao, School of Medicine and Pharmacy, Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, Qingdao, P. R. China.
  • Aowei Xie
    College of Food Science and Engineering, Ocean University of China, Qingdao, P. R. China.
  • Ning Hou
    Guangdong Provincial Key Laboratory of Technique and Equipment for Macromolecular Advanced Manufacturing, School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou 510641, China.
  • Fengjiao Wei
    Marine Biomedical Research Institute of Qingdao, School of Medicine and Pharmacy, Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, Qingdao, P. R. China.
  • Ting Gao
    College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
  • Jiajie Li
    Marine Biomedical Research Institute of Qingdao, School of Medicine and Pharmacy, Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, Qingdao, P. R. China.
  • Xinru Gao
    Marine Biomedical Research Institute of Qingdao, School of Medicine and Pharmacy, Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, Qingdao, P. R. China.
  • Chuanqin Shi
    Center of Translational Medicine, Zibo Central Hospital Affiliated to Binzhou Medical University, Zibo, China.
  • Gaokeng Xiao
    Guangzhou Molcalx Information & Technology ltd. Room 3406, F4, Build 3, Xiaozitiantang, Guangzhou, China.
  • Ximing Xu
    Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China.