Screening and preliminary analysis of antimicrobial peptide genes in Octopussinensis.

Journal: Fish & shellfish immunology
Published Date: Aug 1, 2025

Abstract

Antimicrobial peptides (AMPs) are small molecular peptides that widely exist in organisms to resist external microbial invasion and play a crucial role in the host's immune defense system. Owing to their functions of efficient broad-spectrum killing of pathogenic microorganisms, immune enhancement, and intestinal health improvement, they have emerged as a focal point in research on the immune defense of aquatic animals in recent years. In this study, a total of 105 putative AMP-derived genes from the genome were screened, and seven candidate AMPs were finally identified by analyzing the differential expression results of the hepatopancreas and the white body transcriptomes combined with machine learning algorithms. Furthermore, the seven synthesized antimicrobial peptides were demonstrated to have good antimicrobial activity. Among them, GAP1 and Big Defensin showed the strongest antibacterial activity. GAP1 and Big Defensin exhibited antibacterial activity against four bacteria (Escherichia coli, Vibro parahaemolyticus, Staphylococcus aureus, and Bacillus subtilis) at low concentrations of 5-10 μM and 3.2-12.9 μM respectively. These data will contribute to the development of AMP-based aquatic drugs.

Authors

  • Yuquan Zhou
    State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361021, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China.
  • Zebin Chen
    Department of Pharmacy, Shenzhen Children's Hospital, Shenzhen, 518036, People's Republic of China.
  • Yihua Zou
    State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361021, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China.
  • Yongjie Qin
    State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361021, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China.
  • Yonghua Jiang
    Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, Guangxi, China.
  • Pengfei Zou
    State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, 361021, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China.
  • Jianming Zhang
    School of Computer and Communication Engineering, Changsha University of Science and Technology, Changsha 410114, China.
  • Youfang Zhu
    Putian Municipal Institute of Fishery Science, Putian, 351100, China.
  • Ziping Zhang
    College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: zhangziping@hotmail.com.
  • Yilei Wang
    Department of Psychology, University of Minnesota at Twin Cities.

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