Analytical and experimental solutions for Fourier transform infrared microspectroscopy measurements of microparticles: A case study on Quercus pollen.
Journal:
Analytica chimica acta
Published Date:
Feb 28, 2025
Abstract
BACKGROUND: FTIR microspectroscopy is a popular non-destructive technique for chemical analysis and identification of microparticles, such as microplastics, pollen, spores, microplankton organisms, sediments and microfossils. Unfortunately, measured spectra of microparticles are usually distorted by Mie-type scattering interferents thus hindering the analysis of spectral data. To retrieve chemical absorbance spectra, two different approaches are regularly employed: analytical (application of scatter-correction preprocessing methods), and experimental (measurement in an embedding matrix). The comparative studies of preprocessing spectral strategies are needed to determine pros and cons of these approaches, and when they are most suitable for use.
