Exploration of Crucial miRNA Signatures and Molecular Mechanisms in the System of Muscle-Exosome-Bone: Evidence from Transcriptome Data.

Orthopedics Pathology Transplantation
Journal: ACS omega
Published Date: May 20, 2025

Abstract

Muscles and bones are adjacent in spatial position and closely related in function. Exosomes can achieve communication between donor and receptor cells by carrying molecules, such as miRNAs. Therefore, the purpose of this study is to use exosome-miRNA as a bridge, explore the correlation between clinical manifestations, and use bioinformatics and machine learning to cluster and screen exosome-miRNA sequencing data. In vitro and in vivo experiments were conducted to validate the screened molecules. Three parts were explored to identify miRNAs that play a key regulatory role in the muscle-exosome-bone system. Ultimately, it was found that miR-92a-1-5p may play a crucial role in this system; that is, atrophic muscle cells can inhibit osteogenic differentiation by releasing exosomes carrying miR-92a-1-5p into osteoblasts and targeting .

Authors

  • Bin Wang
    State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, China; New South Wales Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga 2650, Australia. Electronic address: [email protected].
  • Peng He
    Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
  • Xiaowei Liu
    Greater Bay Area Center for Drug Evaluation and Inspection of National Medical Products Administration, Shenzhen 518017, China.
  • Shunjie Wu
    Department of Orthopedics, Rudong People's Hospital, Nantong 226400, China.
  • Xinlin Lu
    Department of Orthopedics, Rudong People's Hospital, Nantong 226400, China.
  • Bin Xu
    Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Keywords

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