Micro-Net: A unified model for segmentation of various objects in microscopy images.

Journal: Medical image analysis
Published Date:

Abstract

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in microscopy images. The proposed network can be used to segment cells, nuclei and glands in fluorescence microscopy and histology images after slight tuning of input parameters. The network trains at multiple resolutions of the input image, connects the intermediate layers for better localization and context and generates the output using multi-resolution deconvolution filters. The extra convolutional layers which bypass the max-pooling operation allow the network to train for variable input intensities and object size and make it robust to noisy data. We compare our results on publicly available data sets and show that the proposed network outperforms recent deep learning algorithms.

Authors

  • Shan E Ahmed Raza
    Division of Molecular Pathology, The Institute of Cancer Research, UK; Department of Computer Science, University of Warwick, UK; Centre for Evolution and Cancer, The Institute of Cancer Research, London, UK. Electronic address: shan.raza@icr.ac.uk.
  • Linda Cheung
    School of Life Sciences, University of Warwick, UK.
  • Muhammad Shaban
    Tissue Image Analytics Lab, Department of Computer Science, University of Warwick, Coventry, United Kingdom.
  • Simon Graham
    Mathematics for Real World Systems Centre for Doctoral Training, University of Warwick, Coventry, CV4 7AL, UK; Department of Computer Science, University of Warwick, UK. Electronic address: s.graham.1@warwick.ac.uk.
  • David Epstein
    Department of Mathematics, University of Warwick, UK.
  • Stella Pelengaris
    School of Life Sciences, University of Warwick, UK.
  • Michael Khan
    School of Life Sciences, University of Warwick, UK.
  • Nasir M Rajpoot