Cereal grain 3D point cloud analysis method for shape extraction and filled/unfilled grain identification based on structured light imaging.

Journal: Scientific reports
Published Date:

Abstract

Cereals are the main food for mankind. The grain shape extraction and filled/unfilled grain recognition are meaningful for crop breeding and genetic analysis. The conventional measuring method is mainly manual, which is inefficient, labor-intensive and subjective. Therefore, a novel method was proposed to extract the phenotypic traits of cereal grains based on point clouds. First, a structured light scanner was used to obtain the grains point cloud data. Then, the single grain segmentation was accomplished by image preprocessing, plane fitting, region growth clustering. The length, width, thickness, surface area and volume was calculated by the specified analysis algorithms for grain point cloud. To demonstrate this method, experimental materials included rice, wheat and corn were tested. Compared with manual measurement results, the average measurement error of grain length, width and thickness was 2.07%, 0.97%, 1.13%, and the average measurement efficiency was about 9.6 s per grain. In addition, the grain identification model was conducted with 25 grain phenotypic traits, using 6 machine learning methods. The results showed that the best accuracy for filled/unfilled grain classification was 90.184%.The best accuracy for indica and japonica identification was 99.950%, while for different varieties identification was only 47.252%. Therefore, this method was proved to be an efficient and effective way for crop research.

Authors

  • Zhijie Qin
    Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
  • Zhongfu Zhang
    College of Engineering, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
  • Xiangdong Hua
    College of Engineering, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
  • Wanneng Yang
    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Hubei Key Laboratory of Agricultural Bioinformatics and College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Xiuying Liang
    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), College of Engineering, Huazhong Agricultural University, Wuhan, China.
  • Ruifang Zhai
    College of Informatics, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
  • Chenglong Huang
    Colleyville Heritage High School, Colleyville, TX, 76034, USA, ²Highland Park High School, Dallas, TX, 75205, USA, ³Department of Clinical Science, The University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.