Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy.

Journal: Physical chemistry chemical physics : PCCP
PMID: 35895081

Abstract

Water suppression is of paramount importance for many biological and analytical NMR spectroscopy applications. Here, we report the design of a new set of binomial-like radio frequency (RF) pulses that elude water irradiation while exciting or refocusing the remainder of the H spectrum. These pulses were generated using a combination of an evolutionary algorithm and artificial intelligence. They display higher sensitivity relative to classical water suppression schemes and tunable water selectivity to avoid suppressing H resonances near the water signal. The broad bandwidth excitation obtained with these RF pulses makes them suitable for several NMR applications at high and ultra-high-field magnetic fields.

Authors

  • V S Manu
    Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 312 Church St. SE, Minneapolis, MN, 55455, USA. vegli001@umn.edu.
  • Cristina Olivieri
    Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 312 Church St. SE, Minneapolis, MN, 55455, USA. vegli001@umn.edu.
  • KowsalyaDevi Pavuluri
    Department of Radiology, Mayo Clinic, Rochester, MN, 55905, USA.
  • Gianluigi Veglia
    Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 321 Church St. SE, Minneapolis, MN 55455, USA.

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