AlphaPulldown-a python package for protein-protein interaction screens using AlphaFold-Multimer.

Journal: Bioinformatics (Oxford, England)
Published Date:

Abstract

SUMMARY: The artificial intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, AlphaFold-Multimer is also used to discover new protein-protein interactions (PPIs). Here, we present AlphaPulldown, a Python package that streamlines PPI screens and high-throughput modelling of higher-order oligomers using AlphaFold-Multimer. It provides a convenient command-line interface, a variety of confidence scores and a graphical analysis tool.

Authors

  • Dingquan Yu
    European Molecular Biology Laboratory Hamburg, Hamburg 22607, Germany.
  • Grzegorz Chojnowski
    European Molecular Biology Laboratory, c/o DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Maria Rosenthal
    Bernhard Nocht Institute for Tropical Medicine, Hamburg 20359, Germany.
  • Jan Kosinski
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.