Automated classification and characterization of the mitotic spindle following knockdown of a mitosis-related protein.

Journal: BMC bioinformatics
PMID: 29297284

Abstract

BACKGROUND: Cell division (mitosis) results in the equal segregation of chromosomes between two daughter cells. The mitotic spindle plays a pivotal role in chromosome alignment and segregation during metaphase and anaphase. Structural or functional errors of this spindle can cause aneuploidy, a hallmark of many cancers. To investigate if a given protein associates with the mitotic spindle and regulates its assembly, stability, or function, fluorescence microscopy can be performed to determine if disruption of that protein induces phenotypes indicative of spindle dysfunction. Importantly, functional disruption of proteins with specific roles during mitosis can lead to cancer cell death by inducing mitotic insult. However, there is a lack of automated computational tools to detect and quantify the effects of such disruption on spindle integrity.

Authors

  • Matloob Khushi
    Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia. mkhushi@uni.sydney.edu.au.
  • Imraan M Dean
    Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia.
  • Erdahl T Teber
    Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia.
  • Megan Chircop
    Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia.
  • Jonathan W Arthur
    Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia.
  • Neftali Flores-Rodriguez
    Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia.

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