Automated stereological image analysis approach of the human placenta: Surface areas and vascularization.

Journal: Placenta
PMID: 37688891

Abstract

Detecting and quantifying surface densities of placental villi and their vasculature adds important information on the development of the placenta under different exposures and pathological conditions. Today, a larger number of samples and tissue areas can be examined using automated Artificial Intelligence-based approaches. Although each image series calls for a particular approach, sharing the methods will help in facilitating reproducibility and comparability. Here we show the protocol of a software-based quantification of vessels (number and area) in villous tissues of human placentas, based on scanned images of full-size placental sections.

Authors

  • Saghi Zafaranieh
    Institute of Human Movement Science, Sport and Health, University of Graz, 8010, Graz, Austria; Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria.
  • Daniel Kummer
    Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria.
  • Mireille N M van Poppel
    Institute of Human Movement Science, Sport and Health, University of Graz, 8010, Graz, Austria.
  • Gernot Desoye
    Department of Obstetrics and Gynaecology, Medical University of Graz, Auenbruggerplatz 14, 8036, Graz, Austria.
  • Berthold Huppertz
    Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria. Electronic address: berthold.huppertz@medunigraz.at.

Keywords

External Resources

Popular Topics
Recent Journals