Svetlana a supervised segmentation classifier for Napari.

Journal: Scientific reports
Published Date:

Abstract

We present Svetlana (SuperVised sEgmenTation cLAssifier for NapAri), an open-source Napari plugin dedicated to the manual or automatic classification of segmentation results. A few recent software tools have made it possible to automatically segment complex 2D and 3D objects such as cells in biology with unrivaled performance. However, the subsequent analysis of the results is oftentimes inaccessible to non-specialists. The Svetlana plugin aims at going one step further, by allowing end-users to label the segmented objects and to pick, train and run arbitrary neural network classifiers. The resulting network can then be used for the quantitative analysis of biophysical phenoma. We showcase its performance through challenging problems in 2D and 3D and provide a comprehensive discussion on its strengths and limits.

Authors

  • Clément Cazorla
    Institut de Mathématiques de Toulouse (IMT), Université de Toulouse, Toulouse, France. clement.cazorla31@gmail.com.
  • Renaud Morin
    Imactiv-3D. Centre Pierre Potier, 1 place Pierre Potier, 31100, Toulouse, France.
  • Pierre Weiss
    Institut de Recherche en Informatique de Toulouse (IRIT), Centre de Biologie Intégrative (CBI), Laboratoire de biologie Moléculaire, Cellulaire et du Développement (MCD), Université de Toulouse, CNRS, Toulouse, France.