[Progress in the application of artificial intelligence-assisted molecular modification of enzymes].
Journal:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
PMID:
38914488
Abstract
Natural enzymes are often difficult to meet the needs of application and research in terms of activity, enantiomer selectivity or thermal stability. Therefore, it is an important task of enzyme engineering to explore efficient molecular modification technologies to improve the properties of such enzymes. The molecular modification technologies of enzymes mainly include rational design, directed evolution, and artificial intelligence-assisted design. Directed evolution and rational design are experiment-driven molecular modification approaches of enzymes and have been successfully applied to enzyme engineering. However, due to the huge space sizes of protein sequences and the lack of experimental data, the current modification methods still face major challenges. With the development of next-generation sequencing, high-throughput screening, protein databases, and artificial intelligence (AI), data-driven enzyme engineering is emerging as a promising solution to these challenges. The AI-assisted statistical learning method has been used to establish a model for predicting the sequence/structure-properties of enzymes in a data-driven manner. Excellent mutant enzymes can be selected according to the prediction results, which greatly improve the efficiency of molecular modification. Considering the application requirements of molecular modification of enzymes, this paper reviews the data acquisition methods and application examples of AI-assisted molecular modification of enzymes, with focuses on the convolutional neural network method for predicting protein thermostability, aiming to provide reference for researchers in this field.