ACS synthetic biology
Dec 21, 2025
Enzymes are biological catalysts that speed up chemical reactions in an eco-friendly way. Precise enzyme design is hindered by vast sequence space and intricate sequence-structure-function interdependencies. To address these challenges, we developed ...
Journal of chemical information and modeling
Dec 17, 2025
Enzyme kinetic parameters, including , , /, and , are critical for guiding applications in enzyme engineering, metabolic modeling, and synthetic biology by providing quantitative information on enzyme activity under various conditions. Experimental d...
Materials horizons
Dec 8, 2025
An augmented strategy for constructing intelligent soft robots includes the transfer of biogenic features from nature to man-made artificial systems serving a range of life-like functions. Inspired by living technology, we have customized macroscale ...
BMC bioinformatics
Nov 26, 2025
BACKGROUND: The functional annotation of uncharacterized microbial enzymes from metagenomic data remains a significant challenge, limiting our understanding of microbial metabolic dynamics. Traditional annotation methods often rely on sequence homolo...
ACS synthetic biology
Oct 28, 2025
The booming artificial intelligence (AI) technology provides an opportunity to precisely carry out design of enzymes and create new biocatalysts with significantly enhanced performance. In the past decade, successful enzyme design cases, although t...
Journal of chemical information and modeling
Oct 14, 2025
Accurate prediction of enzyme kinetic parameters ( and ) is crucial for enzyme rational design and engineering research. Based on a heterogeneous data set encompassing 17,893 and 24,585 records across 8911 enzyme sequences from 7 EC classes and 502...
Journal of chemical information and modeling
Oct 1, 2025
Developing enzymes with desired thermal properties is crucial for a wide range of industrial and research applications, and determining temperature stability is an essential step in this process. Experimental determination of thermal parameters is la...
Journal of agricultural and food chemistry
Aug 11, 2025
Enzymes, recognized for their remarkable catalytic efficiency, play a crucial role in a myriad of biochemical reactions. However, the catalytic performance of natural enzymes frequently does not meet the demands of specific applications. To address t...
International journal of biological macromolecules
Jul 23, 2025
Targeting distal mutations holds promising implications for enzyme engineering. Here, we present an open-source computational workflow designed to explore the functional impact of distal sites, demonstrated on an artificial enzyme built on the widely...
Food chemistry
Jul 17, 2025
Aflatoxins (AFs), potent carcinogenic mycotoxins, pose a major global threat to human health. This review offers an in-depth summary of microorganisms capable of degrading AFs, including bacteria, probiotics, and fungi, and highlights the key enzymes...