Analysis of high-molecular-weight proteins using MALDI-TOF MS and machine learning for the differentiation of clinically relevant Clostridioides difficile ribotypes.

Journal: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
PMID: 39688756

Abstract

PURPOSE: Clostridioides difficile is the main cause of antibiotic related diarrhea and some ribotypes (RT), such as RT027, RT181 or RT078, are considered high risk clones. A fast and reliable approach for C. difficile ribotyping is needed for a correct clinical approach. This study analyses high-molecular-weight proteins for C. difficile ribotyping with MALDI-TOF MS.

Authors

  • Ana Candela
    Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • David Rodríguez-Temporal
    Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Mario Blázquez-Sánchez
    Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón and Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Madrid, Spain.
  • Manuel J Arroyo
    Clover Bioanalytical Software, Av. del Conocimiento, 41, Granada 18016, Spain.
  • Mercedes Marín
    Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Luis Alcalá
    Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón and Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Madrid, Spain.
  • Germán Bou
    Clinical Microbiology Department, Complexo Hospitalario Universitario A Coruña, Institute of Biomedical Research A Coruña (INIBIC), A Coruña, Spain.
  • Belén Rodríguez-Sánchez
    Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Marina Oviaño
    Clinical Microbiology Department, Complexo Hospitalario Universitario A Coruña, Institute of Biomedical Research A Coruña (INIBIC), A Coruña, Spain.

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