Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells.

Journal: BMC biology
PMID: 40350411

Abstract

BACKGROUND: Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-protein complexes known as rod-ring (RR) structures that dissociate when ESCs differentiate. Manual analysis of RR structures from confocal microscopy images, although possible, is not feasible on a large scale due to the quantity of RR structures present in each field of view. To address this analysis bottleneck, we have created a fully automatic RR image classification pipeline to segment, characterise and measure feature distributions of these structures in ESCs.

Authors

  • Samuel T M Ball
    Institute of Life-Course and Medical Sciences, Faculty of Health and Life Sciences, William Henry Duncan Building, University of Liverpool, Liverpool, UK.
  • Meagan J Hennessy
    Institute of Life-Course and Medical Sciences, Faculty of Health and Life Sciences, William Henry Duncan Building, University of Liverpool, Liverpool, UK.
  • Yuhan Tan
    Institute of Life-Course and Medical Sciences, Faculty of Health and Life Sciences, William Henry Duncan Building, University of Liverpool, Liverpool, UK.
  • Kai F Hoettges
    Department of Electrical and Electronic Engineering, Faculty of Science and Engineering, University of Liverpool, Liverpool, UK.
  • Neil D Perkins
    Faculty of Medical Sciences, Biosciences Institute, University of Newcastle, Newcastle, UK.
  • David J Wilkinson
    Institute of Life-Course and Medical Sciences, Faculty of Health and Life Sciences, William Henry Duncan Building, University of Liverpool, Liverpool, UK.
  • Michael R H White
    Faculty of Biology, Medicine and Health, School of Biological Sciences, University of Manchester, Manchester, UK.
  • Yalin Zheng
    Department of Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom.
  • David A Turner
    Institute of Life-Course and Medical Sciences, Faculty of Health and Life Sciences, William Henry Duncan Building, University of Liverpool, Liverpool, UK. david.turner@liverpool.ac.uk.

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