AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing.

Journal: Communications biology
PMID: 40360739

Abstract

Current methods for producing cardiomyocytes from human induced pluripotent stem cells (hiPSCs) using 2D monolayer differentiation are often hampered by batch-to-batch variability and inefficient purification processes. Here, we introduce CM-AI, a novel artificial intelligence-guided laser cell processing platform designed for rapid, label-free purification of hiPSC-derived cardiomyocytes (hiPSC-CMs). This approach significantly reduces processing time without the need for chronic metabolic selection or antibody-based sorting. By integrating real-time cellular morphology analysis and targeted laser ablation, CM-AI selectively removes non-cardiomyocyte populations with high precision. This streamlined process preserves cardiomyocyte viability and function, offering a scalable and efficient solution for cardiac regenerative medicine, disease modeling, and drug discovery.

Authors

  • Prakaimuk Saraithong
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Peyton Krajcarski
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Yukako Kusaka
    Kataoka Corporation, Kyoto, Japan.
  • Moe Yamada
    Kataoka Corporation, Kyoto, Japan.
  • Junichi Matsumoto
    Department of Emergency and Critical Care Medicine, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.
  • Hailey Cunningham
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Sama Salih
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Darby Jones
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Devika Baddhan
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Christian Hausner
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA.
  • Justus Anumonwo
    Center for Arrhythmia Research, Department of Internal Medicine, Division of Cardiovascular Medicine, University of Michigan, Ann Arbor, MI-48109, USA.
  • Anthony Rosenzweig
    Department of Internal Medicine-Cardiology, University of Michigan, Ann Arbor, MI, USA.
  • Mary M Navarro
    StemBioSys, Inc., San Antonio, TX, USA.
  • Luis Villa Diaz
    Departments of Biological Sciences and Bioengineering, Oakland University, Rochester, MI, USA.
  • Joseph Criscione
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
  • Deok-Ho Kim
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Todd J Herron
    Frankel Cardiovascular Regeneration Core Laboratory University of Michigan, Ann Arbor, MI, USA. toddherr@stanford.edu.

Keywords

External Resources

Popular Topics
Recent Journals