AIMC Topic: Cells, Cultured

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Optogenetic neural spheroids excite primary neural network.

Journal of neural engineering
Optical stimulation ofneurons requires prior transfection with light gated ion channels. This additional step brings complexity and requires optimization. Simplification of the process will ease the undertaking of studies on biological neural network...

Evaluating safe infrared neural stimulation parameters: Calcium dynamics and excitotoxicity thresholds in dorsal root ganglia neurons.

Journal of neuroscience methods
BACKGROUND: As a promising neural stimulation technique, infrared neural stimulation (INS) has recently gained significant attention due to its ability to stimulate neuronal activities without needing exogenous agents. NIR light is absorbed by water ...

Impact of dental pulp cells-derived small extracellular vesicles on the properties and behavior of dental pulp cells: an in-vitro study.

BMC oral health
BACKGROUND: Dental pulp cells-derived small extracellular vesicles (DPCs-sEVs) had shown immunomodulatory, anti-inflammatory, and tissue function restorative abilities. Therefore, DPCs-sEVs should be considered as a promising regenerative tool for de...

Repetitive training enhances the pattern recognition capability of cultured neural networks.

PLoS computational biology
Cultured neural networks in vitro have demonstrated the biocomputing capability to recognize patterns. However, the underlying mechanisms behind information processing and pattern recognition remain less understood. Here, we developed an in vitro neu...

Evaluation of the effect of cell freshness on pyrogen detection using a serum-free monocyte-activation test.

PloS one
Pyrogens cause shock symptoms when released into the bloodstream. They are classified into two main categories: endotoxins (lipopolysaccharides [LPS]) and non-endotoxin pyrogens. The monocyte activation test (MAT) is an in vitro assay to detect pyrog...

Effects of aqueous and ethanolic extracts of Chinese propolis on dental pulp stem cell viability, migration and cytokine expression.

PeerJ
BACKGROUND: Propolis is a natural substance produced by honeybees that has various biological properties including, anti-inflammatory, antioxidant and antimicrobial properties. Although previous studies have evaluated the antimicrobial effects of pro...

Functional MRGPRX2 expression on peripheral blood-derived human mast cells increases at low seeding density and is suppressed by interleukin-9 and fetal bovine serum.

Frontiers in immunology
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 express...

Effect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study.

PloS one
AIM: To evaluate in vitro the effect of laser photobiomodulation (PBM) combined or not with 30-nm hydroxyapatite nanoparticles (HANp), on the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) by morphometric analysi...

Evaluating deep learning techniques for optimal neurons counting and characterization in complex neuronal cultures.

Medical & biological engineering & computing
The counting and characterization of neurons in primary cultures have long been areas of significant scientific interest due to their multifaceted applications, ranging from neuronal viability assessment to the study of neuronal development. Traditio...