A robotic platform to screen aqueous two-phase systems for overcoming inhibition in enzymatic reactions.

Journal: Bioresource technology
PMID: 30754004

Abstract

Aqueous two-phase systems (ATPS) can be applied to enzymatic reactions that are affected by product inhibition. In the biorefinery context, sugars inhibit the cellulolytic enzymes in charge of converting the biomass. Here, we present a strategy to select an ATPS (formed by polymer and salt) that can separate sugar and enzymes. This automated and miniaturized method is able to determine phase diagrams and partition coefficients of solutes in these. Tailored approaches to quantify the solutes are presented, taking into account the limitations of techniques that can be applied with ATPS due to the interference of phase forming components with the analytics. The developed high-throughput (HT) platform identifies suitable phase forming components and the tie line of operation. This fast methodology proposes to screen up to six different polymer-salt systems in eight days and supplies the results to understand the influence of sugar and protein concentrations on their partition coefficients.

Authors

  • Bianca Consorti Bussamra
    Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands; Development of Processes and Products (DDPP), University of Campinas, Av. Albert Einstein, 500, 6066 Campinas, Brazil. Electronic address: b.consortibussamra@tudelft.nl.
  • Joana Castro Gomes
    Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands.
  • Sindelia Freitas
    Development of Processes and Products (DDPP), University of Campinas, Av. Albert Einstein, 500, 6066 Campinas, Brazil.
  • Solange I Mussatto
    Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens Lyngby, Denmark. Electronic address: smussatto@biosustain.dtu.dk.
  • Aline Carvalho da Costa
    Development of Processes and Products (DDPP), University of Campinas, Av. Albert Einstein, 500, 6066 Campinas, Brazil. Electronic address: accosta@feq.unicamp.br.
  • Luuk van der Wielen
    Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands; Bernal Institute, University of Limerick, Castletroy, Limerick, Ireland. Electronic address: L.A.M.vanderWielen@tudelft.nl.
  • Marcel Ottens
    Dept. of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.

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